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Cytokine Analysis by Multiplex
Multiplex bead-based assays allow for detection and quantification of multiple secreted cytokines in as little as 25 uL of:
- Tissue culture supernatants
- Serum
- Plasma
- Other bodily fluids
Multiplex technology provides a dynamic, cost-effective alternative to preforming multiple ELISAs.
The expression profile of multiple cytokines is useful for:
- Understanding several inflammatory diseases
- Characterization of antigenic response in the immune system
- Immune profiling
- Screening potential drug candidates.
As a contract research organization (CRO), Xeno Diagnostics provides reliable cytokine quantification and analysis using Luminex multiplex technology.
| Cytokine | Lower LOQ (pg/mL) | Upper LOQ (pg/mL) |
|---|---|---|
IL-2 |
3.01 |
3754 |
| IL-4 | 1.10 | 9806 |
| IL-10 | 1.58 | 9394 |
| IFN-y | 1.99 | 9729 |
| TNFa | 3.09 | 1368 |
For other analytes, please inquire.
Example of multiplex cytokine data:
References:
Defawe OD, Fong Y, Vasilyeva E, Pickett M, Carter DK, Gabriel E, et al. Optimization and qualification of a multiplex bead array to assess cytokine and chemokine production by vaccine-specific cells. J Immunol Methods 2012; 382:117-28.